ATG3 anticorps

N° du produit ABIN349972

Cet anticorps Mouton Polyclonal détecte spécifiquement ATG3 dans WB et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour ATG3 anticorps (ABIN349972)

Antigène: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ATG3 anticorps

Fonction: Sheep antibody to ATG3

Specificité: Specific for ATG3.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur ATG3

Antigène: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Autre désignation: ATG3

Sujet: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3 a protein-conjugating enzyme essential for autophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed with a highest expression in heart skeletal muscle kidney liver and placenta.

UniProt: Q9NT62

Pathways: Autophagy